Hi all, We just split our largest hive. We moved some brood frames and honey to another box after brushing the bees back into the old hive yesterday. We put a queen excluder on the old hive box and then put the new box on top. We did this sine we couldn't find the old queen who could be hiding given how these bees make comb. We've recently seen her and lots of evidence of laying so we're sure she's still there. Nurse bees moved up through the queen excluder covering the brood frames. We took this box off early today and moved it to another location. My question is this: When can we add the new queen we bought to this new box? I've been told to wait 24 hours or they'll kill the queen, but it would be easier to do it late this evening. Would that be too soon?