Insect Innate Immunity Database (IIID): An Annotation Tool for Identifying Immune Genes in Insect Genomes We developed an online tool called the Insect Innate Immunity Database (IIID) to provide an open access resource for insect immunity and comparative biology research (http://www.vanderbilt.edu/IIID). The intuitive web interface allows researchers to search for specific immunity genes by name, retrieve all immunity genes in the database for a particular species, pathway or class, and find putative homologs for a gene of interest using an internal BLAST tool. To facilitate the annotation of innate immunity genes in insects, we initially created an Insect Immunity Database (IIID) composed of the published immune repertoires of four insect models spanning several different orders: Drosophila melanogaster, Diptera , , Anopheles gambiae, Diptera , , Apis mellifera, Hymenoptera , , and Acrythosiphon pisum, Hemiptera . Sequence information was obtained through NCBI for the 105 immunity genes described for Acrythosiphon pisum , 317 genes for Anopheles gambiae , , 379 genes for Drosophila melanogaster , , and 174 genes for Apis mellifera , . The initial IIID was compiled using the immune repertoires of D. melanogaster, A. gambiae, A. pisum, and A. mellifera for a combined total of 975 genes.