I just pulled about 20 drone pupa from there cells. At 10 pupa the mite count was at 1.5 mites per. at 20 the count had fallen to 1 per. I suspect that count is low but don't recall what the number is where I really need to treat. I expect to treat this hive before winter sets in regardless. Jsut hoping someone can give me the number for a mite count on pupa. I can find it for drop count no problem.