i received a phone call last evening from a beek friend of mine in minnesota, so thought i would post this question. the scenario is he has a russian hybrid hive (russian/carni queen) developed from a 5 frame nuc last june. the hive built up well, (2 or 3 deeps) overwintered well, was a strong hive coming into spring. in late april or early may he cleaned off the bottom board and reports he hasnâ€™t seen the queen or eggs since, (and may have reversed). (mia queen kia, due to beekeep error most likely, now beside the point.) later, (about may 6[SUP]th[/SUP] ),he removed a number of frames with swarm cells and varying ages of brood, shakes of bees, to make up a 9 frame deep nuc. that nuc is doing well and has raised itâ€™s own queen. the parent hive has now been queenless since the bottom board cleaning and the bees have failed to requeen themselves, even though they had been given 2 frames of brood and eggs. he wants to requeen the parent hive with a russian hybrid queen from the same producer that sold him the original nuc hives, however these bees are now much older, having been queenless since early may, yet have produced 100 lbs of honey. he reports there are no signs of laying workers. i suspect trying to requeen this hive would be difficult, and a success rate questionable at best, given that these are older bees. he wants to try requeening. i suggested that he place two frames of brood and eggs in (from another hive), near the placement of the queen. lay the queen on the top of the frames and observe the bees behavior, which most likely might tell him how well they well accept her to begin with. place the queen in leaving the cap or piece of tape on the cage(jz bz cage) for 24-48 hours, then remove the cap/tape and let the bees release her. (delayed release). the producer told him essentially above as i did, brushing the bees from the donor hives, and warned of the success rate, but recommended manually releasing her after 2 or 3 days, rather than a delayed release. any thoughts or other suggestions from others i might pass onto him. he has ordered the queen and will be requeening friday or saturday.