I had the state inspector over today. We pulled a sugar shake on three hives. All Wayne's bees. 1..9 year old hive, never treated with anything.... 20 mites. 2..Old hive of Italians given to me this summer and requeened in July with Wayne's bees...16 mites. 3..This year's hive, Started with removal bees and Wayne's bees queen...8 mites. None of these hives have been treated at anytime with anything. I am well satisfied with the results.