First year Beekeeper, and I'm stuck again. I don't know what killed my hive, and I so confused and frustrated trying to figure out what did. Every resource I've read about it doesn't give me enough of an answer to decide. It's like, "so you had these symptoms? Here's twenty things that may have caused it." I'd really like to know if I did something wrong, and if it is safe to reuse the frames, but I'm just so stuck. We have cold, long, winters here, and one of two colonies died around the beginning of February. Most, if not all the bees were dead inside the hive. They had only eaten a small amount of honey, there was no evidence of mice, and I treated for Varroa that autumn. I can't remember the mite count when I treated, and will be keeping track of that in the future. I tested the dead brood with the toothpick method, and based on that don't believe it was AFB. This was my much stronger hive. There was bee defecation in the hive, and clusters of bees head down in cells. There was very little brood; the cluster in the photo is the most I saw. They had a screened bottom board and wrap on. I found the poor Queen on top of the pile of bees. I'm sure I'm forgetting details, so please remind me. I would really appreciate someone else's input!