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Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
Beneficial pollinators viz. Pieris rapae and Mamestra configurata occur on members of the family Brassicaceae while Apis mellifera on Asteraceae members along-with M. persicae. It is presumed that siRNAs may be transferred to the pollinators when they suck the plant sap. To examine any homology between the targeted MySP sequence to its orthologous counterparts in beneficial pollinators, serine protease gene sequences of pollinator insects Apis mellifera, (Accession no. NM_001011584), Tribolium castaneum (Accession no. NM_001170771), Mamestra configurata (Accession no. FJ205442), and Pieris rapae (Accession no. FJ882067) from GenBank were aligned using Clustal W program for generating a phylogenetic tree (Fig. 2).
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046343
Beneficial pollinators viz. Pieris rapae and Mamestra configurata occur on members of the family Brassicaceae while Apis mellifera on Asteraceae members along-with M. persicae. It is presumed that siRNAs may be transferred to the pollinators when they suck the plant sap. To examine any homology between the targeted MySP sequence to its orthologous counterparts in beneficial pollinators, serine protease gene sequences of pollinator insects Apis mellifera, (Accession no. NM_001011584), Tribolium castaneum (Accession no. NM_001170771), Mamestra configurata (Accession no. FJ205442), and Pieris rapae (Accession no. FJ882067) from GenBank were aligned using Clustal W program for generating a phylogenetic tree (Fig. 2).
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046343
